Abstract Danzhi Jiangtang capsule (DJC), a traditional medicinal formula composed of cortex moutan, heterophylly falsestarwort root, unprocessed rehmannia root, oriental waterplantain rhizome, dodder seed and leech, has been used to treat type 2 diabetes in clinic for almost 10 years. In addition, DJC was also confirmed that it could relieved oxidative stress of vascular endothelium in diabetic macroangiopathy. Based on the fact that the DJC could regulated the level of free fatty acids in diabetes patients and the constituents of DJC also possess potential anti-hyperlipidemic effect, thus, the present work aimed to evaluate the anti-hyperlipidemic effect of DJC and further investigated potential molecular mechanism of this action. As in vivo results revealed, DJC could reduced the levels of TC, TG, LDL, increased HDL level as well as decreased the level of ALT and AST of model rats. In addition, DJC also showed significant antioxidant effect by decreasing the level of MDA and increasing the level of SOD and GSH-Px. Furthermore, western blot analysis showed that DJC could up-regulated the expression of phosphorylated JAK2 and STAT3, and down-regulated the expression of SCOC3 protein, which indicated that DJC could regulate the metabolism of lipid via JAK2/STAT3 pathway.

ABSTRACT Proso millet (Panicum miliaceum L.) is important in some Kazakhstan food, this research objective was to develop pre- breeding waxy proso millet resource for breeding new varieties by using phenotyping and marker-assisted selection. The amylose content of the endosperm starch in a collection of 18 proso millet cultivars widely used in northern Kazakhstan were evaluated and ranged from 14.6% to 34.8%. The introduced glutinous millet accessions of PI436626 (Lung Shu 18), PI436625 (Lung Shu 16) were selected to cross with local cultivars (Saratovskoe 6 and Pamyati Bersieva). The potassium iodide (KI) staining method and PCR analysis using the 9bF/15delRB primer were used to identify the waxy trait for selection of the F2-F3 generation. Seeds staining dark blue to black were scored as wild type, while waxy seeds were stained as pinkish or amber to reddish-brown color. The hybridological analysis of inheritance of the “waxy” trait suggested that the segregation ratios for waxy endosperm in the F2 and F3 populations was close to 15:1 of the caryopses with blue and brown colors. PCR analysis with the 9bF/15delRB primer confirmed that this specific primer flanks a 123 bp fragment in amylose samples, while in glutinous samples, this primer promotes the amplification of 108 bp long PCR product (15 nucleotides deletion). Finally, the waxy lines were obtained and could be used for further testing.